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A critical role for the protein kinase PKK in the maintenance of

To circumvent the early lethality of PKK knockout mice we generated conditional PKK knockout mice in which exon 2 of the PKK gene was flanked by loxP sites Fig 1A To generate mice lacking PKK activity specifically in the B cell compartment we crossed the mice carrying floxed PKK alleles PKK fl/fl onto a CD19 Cre transgenic strain which express Cre recombinase under transcriptional

Protein kinase C associated kinase regulates NF κB activation through

PKK interacts with IKKα and IKKβ A crucial regulatory step in the activation of NF κB pathways is the activation of IKK complexes 4 5 19 It was previously reported that activation of an NF κB reporter by PKK overexpression was inhibited by dominant negative mutants of IKKα and IKKβ and that PKK mediated activation of the NF κB reporter was diminished in mouse embryo fibroblasts

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These separations were performed both with 293T cells transfected with wild type PKK as well as with cells transfected with K51R PKK Fig 6 c right panel As depicted in Fig 6c the major Triton X 100 soluble form of PKK migrates at 110 kDa and a minor amount of this underphopshorylated 100 kDa form was also seen in the Triton X 100

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